In Vitro Studies on The Protoporphyrin Uptake and Photosensitivity of Normal Skin Fibroblasts and Fibroblasts From Patients With Erythropoietic Protoporphyria
作者： Albert A Schothorst , Dick. Suurmond , Johans S Ploem
DOI: 10.1111/1523-1747.EP12688381
关键词:
摘要: Fibroblasts derived from patients with erythropoietic protoporphyria (EPP) are not sensitive to violet light and do contain an excess of protoporphyrin (PP). both normal individuals EPP can take up very low concentrations PP culture medium. Cells grown in PP-containing medium showed a gradually increasing but limited uptake also increased sensitivity light. A scanning microfluorometric method has made it possible demonstrate that the is mainly localized perinuclear granules. After exposure light, cells photosensitized by membrane permeability as well reduced reproductive capacity. Both these photodamage effects be repaired during postirradiation incubation at 37°C.
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