An improved high yield method to obtain microsatellite genotypes from red deer antlers up to 200 years old.

摘要: Analysis of DNA from older samples, such as museum specimen, is a promising approach to studying genetics populations and ecological processes across several generations. Here, we present method for extracting high quality nuclear microsatellite analysis antlers red deer (Cervus elaphus). The genotyping individuals was based on nine loci. Because the amount found in high, could reduce sample chemicals used shorten decalcification time comparison other methods. Using these methods, obtained genotypes up 200 years old.