Isolation of variants in phagocytosis of a macrophage-like continuous cell line.

摘要: We have isolated cloned variants in phagocytosis from a continuous murine macrophage-like cell line, J 774.2. A selection procedure against Fc receptor-mediated was devised using IgG-coated SRBC containing toxic drug, tubercidin, as the lethal agent. series of variant clones deficient were isolated. Such occurred at low frequency (approximately 6 X 10(-5)), stable, and appeared to possess receptors. The degree which they defective varied clone clone, yet all clones, able phagocytize latex particles. phagocytic defect some could be corrected by addition 8 Br-cAMP, others, drug without effect. It is likely, therefore, that different are several distinct steps critical phagocytosis.