Regulation of Immunoglobulin Gene Transcription

摘要: Publisher Summary This chapter examines regulation of immunoglobulin gene transcription. It provides an overview cis -acting DNA sequence elements and the trans factors that interact with them, emphasis on are likely to be critical in stage-specific activation genes. Promoters required for transcription genes located 5′ all variable region segments can brought into proximity constant region-associated enhancers by recombination. The most striking feature promoters is presence a conserved octanucleotide motif. Deletion this site heavy chain promoter results not only loss activity transient transfection assays, but complete lymphoid expression transgene mice. analysis suggests their controlled through combinatorial action tissue- (OTF-2, TF-μB) more widely expressed E motif-binding such as E47/E12. An additional level imparted mechanisms adjacent DNA-binding may alter “cofactors” that, forming complex bound factor, affect its particular cell type.