Fetal and neonatal development of human spleen : An immunohistological study

摘要: Localization and immunophenotype of lymphocyte subsets in fetal human spleens were studied by employing a panel monoclonal antibodies (McAb) an immunoperoxidase staining procedure on frozen tissue sections. Spleens varied from 15 weeks gestational age (gestational weeks, gw) to newborn. The white pulp consisted intermediate-sized lymphocytes; no separate compartments could be discerned. Germinal centre development was not observed. Dendritic cells stained for B2, HB5, aC3bR, anti-DRC OKIa, but most cases immunoglobulin. Although low cellular immunity is observed neonates, T showed adult phenotypes proportions comparable the situation; immature OKT6(+) lymphocytes rarely seen. Very few with anti-NK cell antibody Leu7. B all expressed B1, Leu14, T10 strongly positive BA1, mostly very weakly B2 HB5. Almost IgM IgD simultaneously, IgG. IgA-positive absent. At gw considerable number IgM(+) Leu1 staining, this decreased during development. These may represent normal counterpart Leu1(+) B-CLL immunocytic centrocytic malignant lymphomas. After 25 only Altogether, morphology different predominating B-cell subsets, at least until birth. 'immature' splenic precursors subsets. Considering presumed role marginal zone against TI-2 antigens, absence birth main factor defective these antigens neonates.