Both clades of the epidemic KPC-producing Klebsiella pneumoniae clone ST258 share a modified galactan O-antigen type.
作者： Valéria Szijártó , Luis Miguel Guachalla , Katharina Hartl , Cecília Varga , Pallavi Banerjee
DOI: 10.1016/J.IJMM.2015.12.002
关键词:
摘要: Klebsiella pneumoniae ST258 is a globally disseminated, extremely drug resistant, nosocomial clone with limited treatment options. We show that the vast majority of isolates express modified d-galactan-I lipopolysaccharide O-antigen, termed hereinafter as D-galactan-III. The genetic determinant required for galactan-III synthesis was identified distinct operon adjacent to rfb (wb) locus encoding D-galactan-I synthesis. three genes within encode predicted glycosyltransferases. Testing an isogenic transformant pair revealed expression D-galactan-III, in comparison D-galactan-I, conferred improved survival presence human serum. Eighty-three percent more than 200 draft genome sequences currently available carries corresponding and hence these are antigens. A D-galactan-III specific monoclonal antibody (mAb) shown bind extracted LPS from panel isolates. same mAb confirmed accessibility surface staining irrespective capsular antigens expressed by both clades described previously. Based on data, antigen may represent attractive target active passive immunization approaches against K. ST258.
sci-hub.se 参考文章(36)
C Whitfield, M B Perry, L L MacLean, S H Yu, Structural analysis of the O-antigen side chain polysaccharides in the lipopolysaccharides of Klebsiella serotypes O2(2a), O2(2a,2b), and O2(2a,2c). Journal of Bacteriology. ,vol. 174, pp. 4913- 4919 ,(1992) , 10.1128/JB.174.15.4913-4919.1992
Anthony RM Coates, Gerry Halls, Yanmin Hu, Novel classes of antibiotics or more of the same British Journal of Pharmacology. ,vol. 163, pp. 184- 194 ,(2011) , 10.1111/J.1476-5381.2011.01250.X
R F Kelly, C Whitfield, Clonally diverse rfb gene clusters are involved in expression of a family of related D-galactan O antigens in Klebsiella species. Journal of Bacteriology. ,vol. 178, pp. 5205- 5214 ,(1996) , 10.1128/JB.178.17.5205-5214.1996
C Whitfield, J C Richards, M B Perry, B R Clarke, L L MacLean, Expression of two structurally distinct D-galactan O antigens in the lipopolysaccharide of Klebsiella pneumoniae serotype O1. Journal of Bacteriology. ,vol. 173, pp. 1420- 1431 ,(1991) , 10.1128/JB.173.4.1420-1431.1991
B R Clarke, C Whitfield, Molecular cloning of the rfb region of Klebsiella pneumoniae serotype O1:K20: the rfb gene cluster is responsible for synthesis of the D-galactan I O polysaccharide. Journal of Bacteriology. ,vol. 174, pp. 4614- 4621 ,(1992) , 10.1128/JB.174.14.4614-4621.1992
Tomislav Rukavina, Brigita Ticac, Milorad Susa, Nina Jendrike, Stipan Jonjíc, Pero Lucin, Reinhard Marre, Miljenko Doric, Matthias Trautmann, None, Protective effect of antilipopolysaccharide monoclonal antibody in experimental Klebsiella infection. Infection and Immunity. ,vol. 65, pp. 1754- 1760 ,(1997) , 10.1128/IAI.65.5.1754-1760.1997
M Trautmann, M Ruhnke, T Rukavina, T K Held, A S Cross, R Marre, C Whitfield, O-antigen seroepidemiology of Klebsiella clinical isolates and implications for immunoprophylaxis of Klebsiella infections. Clinical and Vaccine Immunology. ,vol. 4, pp. 550- 555 ,(1997) , 10.1128/CDLI.4.5.550-555.1997
Eduardo Martinez, Borja Bartolomé, Fernando de la Cruz, pACYC184-derived cloning vectors containing the multiple cloning site and lacZα reporter gene of pUC8/9 and pUC18/19 plasmids Gene. ,vol. 68, pp. 159- 162 ,(1988) , 10.1016/0378-1119(88)90608-7
John E. Cronan, A family of arabinose-inducible Escherichia coli expression vectors having pBR322 copy control. Plasmid. ,vol. 55, pp. 152- 157 ,(2006) , 10.1016/J.PLASMID.2005.07.001
Martin B. Oleksiewicz, Gábor Nagy, Eszter Nagy, Anti-bacterial monoclonal antibodies: back to the future? Archives of Biochemistry and Biophysics. ,vol. 526, pp. 124- 131 ,(2012) , 10.1016/J.ABB.2012.06.001