Protein kinase C and dopamine transport--2. Effects of amphetamine in vitro.

摘要: Abstract Rats, injected with small doses of amphetamine (0.03–0.1 mg/kg, i.p.), showed an increase in the soluble and a decrease activity particulate protein kinase C (PKC) striatum, while large (0.3–l.0 mg/kg) had opposite effect decreasing increasing PKC. These effects were manifested as change (ftKm) for calcium, without alteration Vmax. They attenuated by pretreatment benztropine, dopamine (DA) uptake blocker α-methyl-p-tyrosine (α-MT), DA synthesis inhibitor. The 0.1mg/kg insensitive to reserpine but antagonists, SCH 23390 or sulpiride. results suggest that changes PKC induced dose mediated activation autoreceptors, through biophase concentration at synapse. In contrast, 1.0 mg/kg on agonists, LY 171555 SKF 38393. were, furthermore, potentiated simultaneous treatment sulpiride, which indicates two drugs act different mechanisms. larger altered transport site.