Structure of laminin substrate modulates cellular signaling for neuritogenesis

摘要: Laminin, a major component of basement membranes, can self-assemble in vitro into typical mesh-like structure, according to mass-action-driven process. Previously, we showed that pH acidification dramatically increased the efficiency laminin self-assembly, practically abolishing the necessity for minimal protein concentration. Here have characterized the morphologies matrices produced in either neutral or acidic conditions and compared their capacities induce neuritogenesis rat embryonic cortical neurons. Although formed buffer presented aggregates heterogeneous morphology, acidic matrix consisted of homogeneous hexagonal sheet-like structure. The latter was comparable to the assembled vivo at inner limiting membrane retina in newborn rats, shown here, secreted by cultivated cells, shown elsewhere. average neurite length neurons plated on acidic matrices 244.9 μm, whereas this value dropped to 104.1 μm. Increased seemed be associated with higher degree neuronal differentiation, since cell proliferation immediately arrested upon plating, neutral matrices, cell number increased six-fold within 24 hours. Investigation of the mechanisms mediating outgrowth each condition revealed the extensive observed involved activation of protein kinase A, moderate was mediated C and/or myosin light-chain kinase. Explants cerebral cortex from P2 rats did not grow laminin substrate but presented extensive migration the acidic matrix. We propose independently of contact assembling mode differentially modulates neuritogenesis neuroplasticity.