Identification of 18S ribosomal DNA genotype of Acanthamoeba from patients with keratitis in North China
作者： Yan Zhang , Xuguang Sun , Zhiqun Wang , Ran Li , Shiyun Luo
DOI: 10.1167/IOVS.03-1073
关键词:
摘要: Purpose To identify the genotype of 18S ribosomal RNA gene (18S rDNA, Rns) Acanthamoeba strains isolated from patients with keratitis in northern China. Methods The genus-specific primers JDP1 and JDP2 were used for amplification amplimer ASA.S1. With DNA PCR sequencing, Rns genotypes identified according to DF3 sequence variances. Results Of all 26 sequences obtained strains, 18 unique (69.2%). those, 17 T4 one was T3. Conclusions Most China T4. results are agreement recent literature.
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Debra A. Schaumberg, Kristin K. Snow, M Reza Dana, The Epidemic of Acanthamoeba Keratitis: Where Do We Stand? Cornea. ,vol. 17, pp. 3- 10 ,(1998) , 10.1097/00003226-199801000-00001
Diane R Stothard, Jill M Schroeder‐Diedrich, Mohammad H Awwad, Rebecca J Gast, Dolena R Ledee, Salvador Rodriguez‐Zaragoza, Chantal L Dean, Paul A Fuerst, Thomas J Byers, None, The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types Journal of Eukaryotic Microbiology. ,vol. 45, pp. 45- 54 ,(1998) , 10.1111/J.1550-7408.1998.TB05068.X
J. Nagington, P.G. Watson, T.J. Playfair, J. Mcgill, BarrieR. Jones, A.D.McG. Steele, Amoebic infection of the eye. The Lancet. ,vol. 2, pp. 1537- 1540 ,(1974) , 10.1016/S0140-6736(74)90285-2
Cherry F Radford, Ordan J Lehmann, John KG Dart, None, Acanthamoeba keratitis: multicentre survey in England 1992-6 British Journal of Ophthalmology. ,vol. 82, pp. 1387- 1392 ,(1998) , 10.1136/BJO.82.12.1387
G. C. Booton, D. J. Kelly, Y.-W. Chu, D. V. Seal, E. Houang, D. S. C. Lam, T. J. Byers, P. A. Fuerst, 18S Ribosomal DNA Typing and Tracking of Acanthamoeba Species Isolates from Corneal Scrape Specimens, Contact Lenses, Lens Cases, and Home Water Supplies of Acanthamoeba Keratitis Patients in Hong Kong Journal of Clinical Microbiology. ,vol. 40, pp. 1621- 1625 ,(2002) , 10.1128/JCM.40.5.1621-1625.2002
D R Ledee, T J Byers, C M Kirkness, J Hay, D V Seal, Acanthamoeba griffini : Molecular characterization of a new corneal pathogen Investigative Ophthalmology & Visual Science. ,vol. 37, pp. 544- 550 ,(1996)
J. M. Schroeder, G. C. Booton, J. Hay, I. A. Niszl, D. V. Seal, M. B. Markus, P. A. Fuerst, T. J. Byers, Use of Subgenic 18S Ribosomal DNA PCR and Sequencing for Genus and Genotype Identification of Acanthamoebae from Humans with Keratitis and from Sewage Sludge Journal of Clinical Microbiology. ,vol. 39, pp. 1903- 1911 ,(2001) , 10.1128/JCM.39.5.1903-1911.2001
REBECCA J. GAST, DOLENA R. LEDEE, PAUL A. FUERST, THOMAS J. BYERS, Subgenus Systematics of Acanthamoeba: Four Nuclear 18S rDNA Sequence Types Journal of Eukaryotic Microbiology. ,vol. 43, pp. 498- 504 ,(1996) , 10.1111/J.1550-7408.1996.TB04510.X