Using in situ hybridisation to localize renal gene expression in tissue sections.

摘要: The basics of in situ hybridization have been widely applied to a diverse range situations where we need localize the distribution nucleic acids. Advances other molecular techniques such as advent gene microarrays has not diminished significance hybridization, but rather highlight importance being able identify topology expression. In offers degree precision that is unavailable with techniques. This chapter outlines used examine spatial expression kidney using complementary RNA (cRNA) probes both radioactive and non-radioactive labels.