Rapid Selection and Proliferation of CD133(+) Cells from Cancer Cell Lines: Chemotherapeutic Implications

摘要: Cancer stem cells (CSCs) are considered a subset of the bulk tumor responsible for initiating and maintaining disease. Several surface cellular markers have been recently used to identify CSCs. Among those is CD133, which expressed by hematopoietic progenitor as well embryonic various cancers. We isolated cultured CD133 positive [CD133(+)] from cancer cell lines using NASA developed Hydrodynamic Focusing Bioreactor (HFB) (Celdyne, Houston, TX). For comparison, another bioreactor, rotary culture system (RCCS) manufactured Synthecon (Houston, TX) was used. Both HFB RCCS bioreactors simulate aspects hypogravity. In our study, increased CD133(+) growth compared vessel normal gravity control. observed (+)15-fold proliferation fraction with that were 7-days at optimized conditions. The instead yielded (−)4.8-fold decrease in CD133(+)cellular respect after culture. Interestingly, we also found hypogravity environment greatly sensitized cells, normally resistant chemo treatment, become susceptible chemotherapeutic agents, paving way less toxic more effective treatment patients. To be able test efficacy cytotoxic agents vitro prior their use clinical setting on may pave strategies This could an important advancement therapeutic options oncologic patients, allowing targeted personalized chemotherapy regimens higher response rates.