CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide

作者: Marie K. Schwinn , Thomas Machleidt , Kris Zimmerman , Christopher T. Eggers , Andrew S. Dixon

DOI: 10.1021/ACSCHEMBIO.7B00549

关键词:

摘要: … Genome editing with CRISPR/Cas9 … luminescence through high affinity complementation (K D = 700 pM) with an 18 kDa subunit derived from NanoLuc (LgBiT). Using CRISPR/Cas9, …

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