Quantification of fetal antigen 2 (FA2) in supernatants of cultured osteoblasts, normal human serum, and serum from patients with chronic renal failure

摘要: Fetal antigen 2 (FA2) was found within the cytoplasm of osteoblasts and in osteoid material, a bone biopsy with morphological changes renal osteodystrophy. An ELISA technique for FA2 quantification developed, specificity confirmed by comparison electroimmunoassay. The intra- interassay coefficient variations (%) were 8.3 9.7 respectively, detection limit 0.0004 arbitrary units FA2/1 using second-trimester human amniotic fluid as reference (1 AU FA2/1). detected serum-free supernatants from osteoblast cultures. Following size chromatography, distribution (two peaks eluted corresponding to Mw 30 kDa 100 kDa) serum patient chronic failure complicated secondary hyperparathyroidism identical. Probably due limit, only one peak fraction (30 seen normal serum. Significantly greater concentrations sera (n = 14) patients (median: 11.9 mAU FA2/1; range: 5.2-49.0 FA2/1) compared healthy individuals 23) 4.1 range 2.4-9.4 (P less than 0.00001). A close correlation between alkaline phosphatase (R(s) 0.761; P 0.006), c-terminal PTH 0.872; 0.003) intact PTH(1-84) 0.904; 0.011) haemodialysis patients. These data indicate that is synthesized may represent new marker metabolic changes.