NOTCH3 Expression Is Induced in Mural Cells Through an Autoregulatory Loop That Requires Endothelial-Expressed JAGGED1

摘要: Endothelial cells and mural (smooth muscle cells, pericytes, or fibroblasts) are known to communicate with one another. Their interactions not only serve support fully functional blood vessels but also can regulate vessel assembly differentiation maturation. In an effort better understand the molecular components of this heterotypic interaction, we used a 3D model angiogenesis screened for genes, which were modulated by coculturing these 2 different cell types. doing so, discovered that NOTCH3 is gene whose expression robustly induced in endothelial cells. Knockdown small interfering RNA revealed necessary endothelial-dependent differentiation, whereas overexpression sufficient promote smooth expression. Moreover, contributes proangiogenic abilities cocultured Interestingly, found depe...