Selective regulation of apical endocytosis in polarized Madin-Darby canine kidney cells by mastoparan and cAMP.
作者： B van Deurs , P K Holm , P Eker , K Sandvig
DOI: 10.1016/S0021-9258(17)32353-0
关键词:
摘要: We here demonstrate that mastoparan, fluoride, forskolin, cholera toxin, and 8-bromoadenosine-3'-5'-cyclic monophosphate all selectively stimulate apical endocytosis of the protein toxin ricin without increasing uptake at basolateral side polarized Madin-Darby canine kidney cells. Activation adenylyl cyclase an increased level cAMP seem to increase by a clathrin-independent mechanism since stimulation occurred even when clathrin-dependent pathway was blocked low cytosolic pH. The data suggest mastoparan stimulates interacting with heterotrimeric G proteins, also this appears be clathrin independent transferrin strongly inhibited after brefeldin A-induced missorting receptor pole cell. In addition, stimulated clathrin-mediated acidification cytosol. Furthermore, we provide evidence for existence on both surface control Our results epithelial cells can regulated physiological signal.
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