Cellular evaluation of diselenonicotinamide (DSNA) as a radioprotector against cell death and DNA damage

摘要: Diselenonicotinamide (DSNA), a synthetic organoselenium compound, was evaluated for its radioprotective effect in cellular models. A clonogenic assay Chinese Hamster Ovary (CHO) cells and an apoptosis murine splenic lymphocytes indicated that pre-treatment with DSNA at concentration of 25 μM significantly protected them from radiation-induced cell death. Upon irradiation (1-12 Gy), dose-response studies were carried out under similar treatment conditions, dose modification factor (DMF) estimated to be 1.26. Furthermore, showed effect, even when administered after exposure radiation. Mechanistic investigation revealed increased the intracellular levels GPx GSH irradiated cells. In line this observation, addition pharmacological inhibitor cycle, abrogated activity DSNA. The also complemented by ability prevent DNA damage as monitored micronucleus γ-H2AX assays. did not show much change expressions Nrf2 dependent genes (γ-GCL HO-1), but presence against death damage. Additionally, ATRA inhibited DSNA-mediated up-regulation repair gene RAD51, suggesting possible involvement basal anti-genotoxic conclusion, present study demonstrates radioprotection compound containing nutritionally important moieties like selenium nicotinamide.