MSH receptor expression and the relationship to melanogenesis and metastatic activity in B16 melanoma.

摘要: In this study we have compared the effects of different pro-opiomelanocortin (POMC) peptides on melanogenesis and metastasis their relationship to MSH receptor expression in B16F1 melanoma cells. All peptides, apart from beta-endorphin, increased order potency was Nle4DPhe7-alpha-MSH greater than alpha-MSH ACTH[1-39] des-acetyl ACTH[1-24]. A similar found for metastasis, except ACTH [1-24], which had a relatively effect metastasis. These findings suggest that are mediated via same receptor. The results ligand binding studies also indicated presence single with KD value 62 +/- 16pM. This consistent crosslinking using [125I] produced 50-55 kD band analysis by SDS-PAGE. However, relative affinities measured displacement [125I]-Nle4DPhe7-alpha-MSH, did not closely correlate potencies stimulating suggests activation subsequent biological response is determined solely affinity.