作者: Marta Llopis , Maria Antolin , Monica Carol , Natalia Borruel , Francesc Casellas
DOI: 10.1002/IBD.20736
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摘要: Background: The interaction of commensal bacteria with the intestinal immune system is an essential factor in development inflammatory bowel disease (IBD). study isolated bacteria's effects on mucosal response might be relevant for a better understanding pathophysiological mechanisms IBD. Methods: We investigated responses to signals from Escherichia coli ATCC 35345 and probiotic Lactobacillus casei DN-114 001 Crohn's (CD) mucosa. Ileal specimens were obtained during surgery CD patients. Mucosal explants incubated L. or its genomic DNA; TNF-α, IFN-γ, IL-2, IL-6, IL-8, CXCL1 measured supernatant. Second, tissue expression key proinflammatory cytokines (IL-6, TGF-β, IL-23p19, IL-12p35, IL-17F), chemokines (IL-8, CXCL1, CXCL2) was evaluated after incubation E. coli. Finally, combination experiments carried out by incubating both strains at different timepoints. Results: Live significantly decreased secretion mucosa, but effect not reproduced DNA. live downregulated did modify IL-17F. In contrast, upregulated all these cytokines. Interestingly, revealed ability prevent counteract coli. Conclusions: can inflamed mucosa specific downregulation mediators. (Inflamm Bowel Dis 2008)