Lactobacillus casei downregulates commensals' inflammatory signals in Crohn's disease mucosa.

作者: Marta Llopis , Maria Antolin , Monica Carol , Natalia Borruel , Francesc Casellas

DOI: 10.1002/IBD.20736

关键词:

摘要: Background: The interaction of commensal bacteria with the intestinal immune system is an essential factor in development inflammatory bowel disease (IBD). study isolated bacteria's effects on mucosal response might be relevant for a better understanding pathophysiological mechanisms IBD. Methods: We investigated responses to signals from Escherichia coli ATCC 35345 and probiotic Lactobacillus casei DN-114 001 Crohn's (CD) mucosa. Ileal specimens were obtained during surgery CD patients. Mucosal explants incubated L. or its genomic DNA; TNF-α, IFN-γ, IL-2, IL-6, IL-8, CXCL1 measured supernatant. Second, tissue expression key proinflammatory cytokines (IL-6, TGF-β, IL-23p19, IL-12p35, IL-17F), chemokines (IL-8, CXCL1, CXCL2) was evaluated after incubation E. coli. Finally, combination experiments carried out by incubating both strains at different timepoints. Results: Live significantly decreased secretion mucosa, but effect not reproduced DNA. live downregulated did modify IL-17F. In contrast, upregulated all these cytokines. Interestingly, revealed ability prevent counteract coli. Conclusions: can inflamed mucosa specific downregulation mediators. (Inflamm Bowel Dis 2008)

参考文章(38)
R G Crystal, C Saltini, P Pinkston, J Müller-Quernheim, Corticosteroid therapy suppresses spontaneous interleukin 2 release and spontaneous proliferation of lung T lymphocytes of patients with active pulmonary sarcoidosis. Journal of Immunology. ,vol. 139, pp. 755- 760 ,(1987)
Anne R. Gocke, Petra D. Cravens, Li-Hong Ben, Rehana Z. Hussain, Sara C. Northrop, Michael K. Racke, Amy E. Lovett-Racke, T-bet Regulates the Fate of Th1 and Th17 Lymphocytes in Autoimmunity Journal of Immunology. ,vol. 178, pp. 1341- 1348 ,(2007) , 10.4049/JIMMUNOL.178.3.1341
H Ochman, R K Selander, Standard reference strains of Escherichia coli from natural populations. Journal of Bacteriology. ,vol. 157, pp. 690- 693 ,(1984) , 10.1128/JB.157.2.690-693.1984
Meng-Tsung Tien, Stephen E. Girardin, Béatrice Regnault, Lionel Le Bourhis, Marie-Agnès Dillies, Jean-Yves Coppée, Raphaëlle Bourdet-Sicard, Philippe J. Sansonetti, Thierry Pédron, Anti-Inflammatory Effect of Lactobacillus casei on Shigella-Infected Human Intestinal Epithelial Cells Journal of Immunology. ,vol. 176, pp. 1228- 1237 ,(2006) , 10.4049/JIMMUNOL.176.2.1228
Ascensi�n Marcos, Julia W�rnberg, Esther Nova, Sonia G�mez, Alberto Alvarez, Roc�o Alvarez, Jos� A. Mateos, Jos� M. Cobo, The effect of milk fermented by yogurt cultures plus Lactobacillus casei DN-114001 on the immune response of subjects under academic examination stress European Journal of Nutrition. ,vol. 43, pp. 381- 389 ,(2004) , 10.1007/S00394-004-0517-8
Ivan J Fuss, Christoph Becker, Zhiqiong Yang, Catherine Groden, Ronald L Hornung, Frank Heller, Markus F Neurath, Warren Strober, Peter J Mannon, Both IL‐12p70 and IL‐23 are synthesized during active Crohn's disease and are down‐regulated by treatment with anti‐IL‐12 p40 monoclonal antibody Inflammatory Bowel Diseases. ,vol. 12, pp. 9- 15 ,(2006) , 10.1097/01.MIB.0000194183.92671.B6
Ludivine Chapat, Karine Chemin, Bertrand Dubois, Raphaëlle Bourdet-Sicard, Dominique Kaiserlian, Lactobacillus casei reduces CD8+ T cell-mediated skin inflammation. European Journal of Immunology. ,vol. 34, pp. 2520- 2528 ,(2004) , 10.1002/EJI.200425139
Carsten Schmidt, Thomas Giese, Bianca Ludwig, Ina Mueller-Molaian, Thomas Marth, Stefan Zeuzem, Stefan C. Meuer, Andreas Stallmach, Expression of Interleukin-12-Related Cytokine Transcripts in Inflammatory Bowel Disease: Elevated Interleukin-23p19 and Interleukin-27p28 in Crohn's Disease But Not in Ulcerative Colitis Inflammatory Bowel Diseases. ,vol. 11, pp. 16- 23 ,(2005) , 10.1097/00054725-200501000-00003
Karen Manon Lammers, Patrizia Brigidi, Beatrice Vitali, Paolo Gionchetti, Fernando Rizzello, Elisabetta Caramelli, Diego Matteuzzi, Massimo Campieri, Immunomodulatory effects of probiotic bacteria DNA: IL-1 and IL-10 response in human peripheral blood mononuclear cells. Fems Immunology and Medical Microbiology. ,vol. 38, pp. 165- 172 ,(2003) , 10.1016/S0928-8244(03)00144-5
Humberto Jijon, Jody Backer, Hugo Diaz, Helen Yeung, David Thiel, Conor McKaigney, Claudio De Simone, Karen Madsen, DNA from probiotic bacteria modulates murine and human epithelial and immune function Gastroenterology. ,vol. 126, pp. 1358- 1373 ,(2004) , 10.1053/J.GASTRO.2004.02.003