Genetic control of the cell division cycle in yeast: III. Seven genes controlling nuclear division☆

摘要: Temperature-sensitive mutations in seven genes (cdc 2, 6, 7, 9, 13, 14, and 15) of Saccharomyces cerevisiae confer a defect nuclear division. Following shift from the permissive to restrictive temperature, asynchronous populations these mutant cells accumulate uniformly Depending upon particular gene defect, mutants appear terminate development at an early stage division 2), medial 13), or late 15). Experiments employing temperature shifts, time-lapse photomicroscopy, synchronous cultures permitted determination time cell cycle which each thermolabile products completes its function (the execution point) for ensuing These studies reveal program activity required that commences beginning cycle. Subsequent block strains do not undergo separation bud initiation. Apparently, is normally prerequisite latter two processes.