Membrane properties of rat embryonic multipotent neural stem cells

摘要: We have characterized several potential stem cell markers and defined the membrane properties of rat fetal (E10.5) neural cells (NSC) by immunocytochemistry, electrophysiology microarray analysis. Immunocytochemical analysis demonstrates specificity expression Sox1, ABCG2/Bcrp1, shows that nucleostemin labels both progenitor populations. NSCs, like hematopoietic cells, express high levels aldehyde dehydrogenase (ALDH) as assessed Aldefluor labeling. Microarray 96 transporters channels showed Glucose transporter 1 (Glut1/Slc2a1) is unique to NSCs or other differentiated cells. Electrophysiological examination respond acetylcholine its agonists, such nicotine muscarine. low tetrodotoxin (TTX) sensitive insensitive sodium calcium while expressing at least three kinds potassium channels. find gap junction communication mediated connexin (Cx)43 Cx45, essential for NSC survival proliferation. Overall, our results show exhibit a signature can be used determine their location assess ability environment.