Proteomics of Galápagos Marine Iguanas Links Function of Femoral Gland Proteins to the Immune System.

摘要: Communication between individuals via molecules, termed chemosignaling, is widespread among animal and plant species. However, we lack knowledge on the specific functions of substances involved for most systems. The femoral gland an organ that secretes a waxy substance in chemical communication lizards. Although lipids volatile secreted by glands have been investigated several biochemical studies, protein composition secretions remain completely unknown. Applying proteomic approach, provide first attempt to comprehensively characterize from Galapagos marine iguana. Using samples organs, iguana proteome was assembled next-generation sequencing MS, resulting 7513 proteins. Of these, 4305 proteins were present gland, including keratins, small serum proteins, fatty acid-binding Surprisingly, no with discernible roles partner recognition or inter-species could be identified. did find direct associations innate immune system, lysozyme C, antileukoproteinase (ALP), pulmonary surfactant (SFTPD), galectin (LGALS1) suggesting function as important barrier infection. Furthermore, report novel anti-microbial peptides show similar action against Escherichia coli Bacillus subtilis such oncocin, peptide known its effectiveness Gram-negative pathogens. This proteomics data set valuable resource future functional analysis demonstrates also perform system.