Excessive Treadmill Training Enhances Brain-Specific MicroRNA-34a in the Mouse Hippocampus.

摘要: Background: An imbalance between total training load and recovery may cause overtraining (OT). The purpose of the present study was to verify effects OT on expression brain-derived neurotrophic factor (BDNF), its receptor tropomyosin kinase B (TrkB) p75 dynamic patterns brain-specific miR-34a miR-124 or inflammation-related miR-21 miR-132 in mouse hippocampus. Method: Eight weeks old C57BL/6J mice were randomly assigned control (CON), normal (NT) groups. 8-week protocol applied evaluate phenotype endurance (incremental test, ILT) cognitive capacity (Morris water maze test). We used qRT-PCR immunoblotting detect changes molecular level hippocampal samples. Result: Compared with CON, both NT decreased bodyweight after training. After training, increased exhaustion velocity (EV) while EV lower than NT. Mice escape latency CON. percentage time spent probe quadrant number crossing platform times higher CON OT. BDNF, TrkB mRNA levels only exhibited protein BDNF compared In addition, phosphorylation Only Moreover, found be negatively correlated increase accompanied by a decrease performance. Conclusion: summary, training-evoked help improve performance, whereas this conditioning is lost potentially mediated reflect performance