The Polycystic Kidney Disease-1 Protein, Polycystin-1, Binds and Activates Heterotrimeric G-Proteinsin Vitro
作者： Stephen C. Parnell , Brenda S. Magenheimer , Robin L. Maser , Carolyn A. Rankin , Abdelkrim Smine
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摘要: … previously washed with PBS containing 1 mM DTT, 0.1% Triton X-100 (PBS wash buffer), … PBS wash buffer, and the GST-fusion proteins were eluted with five 1 ml aliquots of PBS wash …
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