MMP-7 is involved in the aging of primary human mammary epithelial cells (HMEC).

摘要: Abstract Primary cultures of human mammary epithelial cells underwent significant morphological and functional changes during the aging process between passage 12 (P12) 16 (P16). Concomitant with a progressive expression senescence-associated β-galactosidase as marker, restructured their attachment, increased in size ceased to divide. Young HMEC until P11 demonstrated nearly 100% distinct adhesion molecules such CD24, integrin β1 (CD29) CD44 similar tumor cell line MCF-7. In parallel aging-associated alterations adhesion, CD24 dropped senescent P16 HMECs. However, levels CD29 remained unchanged process. The tumor-associated Muc-1 (CD227), which was expressed about tumorigenic MCF-7 cells, detectable 51% young declined 37% aged P16. association remodeling shape, matrix metalloproteinases including MMP-7 markedly decreased HMEC. contrast, MMP-1, MMP-2 MMP-9 indicating possible role Indeed, down-modulation by RNAi revealed significantly elevated G2/M cycle arrest 2- 3-fold enhanced compared control siRNA transfectants HMEC, respectively. Together, these findings suggested that decreasing contributes accelerated cells.