Studies of enterobacterial lipopolysaccharides; effects of heat and chemicals on erythrocyte-modifying, antigenic, toxic and pyrogenic properties.

摘要: The effects of physical and chemical agents on the biological properties purified enterobacterial lipopolysaccharides have been studied. following observations made: 1. 1. untreated are markedly effective as antibody neutralizing antigens. They possess a high degree toxicity pyrogenicity, but they only limited erythrocyte-modifying capacity. 2. 2. Treatment these with heat or sodium hydroxide for short periods time results in an increase their capacity to modify erythrocytes without alteration antigenicity (antibody-neutralizing potency) pyrogenicity. longer intervals does not affect antibody-neutralizing capacity, marked loss pyrogenicity. 3. 3. Oxidation Escherichia coli lipopolysaccharide periodate yields compound low this substance neutralize is almost completely abolished. 4. 4. A degraded, lipid-free polysaccharide component obtained from Salmonella abortus equi exhibited reduced antibodycombining when compared original product; treatment fails produce any capacity. 5. 5. Red blood cells which modified heated treated specifically absorb homologous bacterial antibodies. Erythrocytes contact equivalent amounts antigens do not. 6. 6. study indicate that various activities affected differentially by different agents. 7. 7. significance data has discussed particular reference relationship physical-chemical characteristics components compounds.