Glycoprotein synthesis in the adult rat pancreas
作者： Robert A. Ronzio
DOI: 10.1016/0003-9861(73)90518-3
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摘要: Abstract Golgi-rich fractions were prepared from homogenates of adult rat pancreas by discontinuous gradient centrifugation. These characterized stacks cisternae associated with large, irregular vesicles and relatively free rough microsomes, mitochondria, zymogen granules. The contained 50% the UDP-galactose: glycoprotein galactosyltransferase activity; specific activity was 12-fold greater than homogenate. Such represented 2+ -adenosine triphosphatase. Zymogen granules extracted 0.2 m NaHCO3, pH 8.2, membranes isolated could not be detected in granule membranes, while Golgi 25-fold At least 35 polypeptide species polyacrylamide gel electrophoresis 1% sodium dodecylsulfate. ranged molecular weight 12,000 to
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