Polyamines influence morphogenesis and caffeine biosynthesis in in vitro cultures of Coffea canephora P. ex Fr.

作者: Vinod Kumar , P. Giridhar , A. Chandrashekar , G. A. Ravishankar

DOI: 10.1007/S11738-007-0110-X

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摘要: The influence of polyamines, polyamine inhibitors and ethylene were tested in Coffea canephora for vitro morphogenetic response caffeine biosynthesis. produced non-embryogenic embryogenic calli. Somatic embryos only from the callus. Endogenous pools estimated these tissues. subjected to secondary embryogenesis under putrescine, silver nitrate specific Estimation endogenous total polyamines revealed that callus contained 11-fold more spermine 3.3-fold higher spermidine when compared Incorporation resulted 58% explant control with 42% response. 65% embryogenesis. biosynthetic pathway DFMO DFMA 83% reduction concomitant increase levels by two-fold as control. These results have clearly demonstrated play a crucial role

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