Fabrication and Characterization of Knitted Silk-Based Composite Scaffold for Bone-Ligament-Bone (B-L-B) Graft

摘要: The Anterior Cruciate Ligament (ACL) is one of the four major ligaments knee. Owing to its central position and key role in load bearing flexing knee, it known be most widely injured body. Scientists have been trying engineer artificial by using novel scaffolds as a replacement beyond repair, but failed post-operative recovery stages due inability ligament-alone graft satisfactorily integrate with bone provide adequate insertional strength. This was found lack formation specialized fibrocartilaginous tissue ‘enthesis’ at junction between ligament. current project has focused on fabrication silk-based knitted scaffold which comprises three compartments, each for bone, ligament enthesis tissues. With motive differentiate MSCs into fibrocartilage lineages compartment amidst mature cell lines, an array methods were adopted surface modification compartmentalized silk bio-polymers such solution, chitosan, polyvinyl alcohol hydroxyapatite appropriate compartments. compartments reserved line culture. various used coatings polymers over knitted-silk alternate soaking, plain coating freeze-drying. characterized parameters water absorption, biodegradability, tensile strength, purity coatings, biocompatibility. It concluded that multi-compartment based developed house novelty engineering bridge component i.e. enthesis.