Responses of microglia in vitro to the gram-positive bacterial component, lipoteichoic acid

摘要: An increase in incidence and severity of gram-positive infections has emerged the past decade. In this regard, attention been focused recently on immune responses microglial cells central nervous system to bacteria. The underlying immunological cellular events activation induced by specific bacterial toxin bacteria, however, have not yet clarified fully. This study reports that a simple cell wall product, lipoteichoic acid (LTA), derived from bacteria (Staphylococcus aureus) could trigger vitro. Microglia challenged with LTA showed intense ruffling plasma membrane form lamellipodia or rounded up forming aggregates. MTT assay Western blot analysis anti-proliferating nuclear antigen antibody significant proliferation may be at later phases treatment low doses but early period high dose. Concentrated also caused apoptotic death cultured microglia showing fragmented nuclei increased expression annexin V caspase 3. response LTA, isolated inducible nitric oxide synthase major histocompatibility complex class II antigen. Microglial receptors such as CD14 molecule, complement receptor type 3, macrophage scavenger were upregulated concurrently. conclusion, staphylococcal can exert an immunomodulatory effect morphology, cycle, immunomolecules, including its receptors.