Differential expression profiles of microRNAs between breast cancer cells and mammary epithelial cells

摘要: BACKGROUND AND OBJECTIVE Aberrant expression of microRNAs (miRNAs) is involved in the development breast cancer. This study was to explore differential miRNAs between cancer cells and mammary epithelial cells, predict potential targets differentially expressed miRNAs. METHODS Human MCF-7 normal human HBL-100 were cultured. The total RNA isolated examined. profiles detected with a miRNA microarray chip compared. Array results confirmed by real-time polymerase chain reaction (PCR). Potential mRNA predicted MiRanda, TargetScan, PicTar, DIANA-microT software. RESULTS purification from relatively high. According microarray-based screening, 173 cancer-related identified. Of miRNAs, 113 up-regulated 60 down-regulated (P<0.01). Real-time PCR showed that mir-18a mir-195 highly cells. Sixty-eight target genes mir-200b informatics analysis. CONCLUSIONS are obtained. Some may be pathogenesis regulating their genes.