MicroRNA-19 contributes to the malignant phenotypes of osteosarcoma in vitro by targeting Pax6

摘要: This study was conducted to detect the expression of miR-19 and Pax6 (Paired box protein 6) in human osteosarcoma cells effects on biological characteristics cells. Quantitative real-time polymerase chain reaction used normal osteoblasts (hFOB 1.19) cell lines (U2OS, Saos-2, MG-63). Results showed that significantly upregulated compared with hFOB 1.19 cells, while messenger RNA downregulated. defined as target gene which validated by luciferase reporter analysis. indicated had an interaction 3'-untranslated region. At same time, decreased MG-63 transfected mimic notably enhanced inhibitor. These data suggested a The behavior were determined 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, flow cytometry, Transwell assay. downregulation inhibited viability, reduced percentage S phase number passing through chamber, increased apoptotic Western blot analysis inhibition epithelial proteins (E-cadherin β-catenin) mesenchymal (Vimentin), extracellular signal-regulated kinase, phosphorylated kinase MiR-19 inhibitor small interfering simultaneously into from assay demonstrated could reverse induced expression. Based these findings, it miR-19, negatively regulated Pax6, can promote malignant phenotypes via activation signaling pathways. Therefore, miR-19/Pax6 may offer potential for use treatment osteosarcoma.