Estimation of free radical-quenching activity of leaf pigment extracts.
作者： Marino B. Arnao , Antonio Cano , Jes�s F. Alcolea , Manuel Acosta
DOI: 10.1002/PCA.571
关键词:
摘要: The chromogen 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) radical (ABTS.+) can be directly generated by the enzymatic system formed hydrogen peroxide and horseradish peroxidase in ethanolic media. reaction is time- concentration-dependent, ABTS.+ shows excellent stability. method an adaptation of decolouration previously reported (Cano et al. 1998. Phytochem Anal 9:196-202), which was used for estimating anti-oxidant activity aqueous When assay described here to quantify two anti-oxidants, Trolox (an analogue vitamin E) beta-carotene, latter had 2.5 times better capacity than at same concentration. free quenching (anti-oxidant activity) leaf pigment extracts measured related carotenoid contents barley, oat citrus. possibilities using this experimental approach evaluate status photoprotective pigments are discussed.
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