Mammalian Mg2+-independent Phosphatidate Phosphatase (PAP2) Displays Diacylglycerol Pyrophosphate Phosphatase Activity
作者： Deirdre A. Dillon , Xiaoming Chen , Geri Marie Zeimetz , Wen-I. Wu , David W. Waggoner
关键词:
摘要: Recent studies indicate that the metabolism of diacylglycerol pyrophosphate (DGPP) is involved in a novel lipid signaling pathway. DGPP phosphatases (DGPP phosphohydrolase) fromSaccharomyces cerevisiae and Escherichia colicatalyze dephosphorylation to yield phosphatidate (PA) then catalyze PA diacylglycerol. We demonstrated Mg2+-independent form phosphatase (PA phosphohydrolase, PAP2) purified from rat liver catalyzed DGPP. This reaction was Mg2+-independent, insensitive inhibition byN-ethylmaleimide bromoenol lactone, inhibited by Mn2+ ions. PAP2 exhibited high affinity for (Km = 0.04 mol %). The specificity constant (Vmax/Km) 1.3-fold higher than PA. ability dephosphorylate PA, Like PAP2, activity S. cerevisiaewas lyso-PA, sphingosine 1-phosphate, ceramide 1-phosphate. Mouse showed homology fromS. E. coli, especially localized regions constitute sequence motif. Collectively, our work indicated member family includes coli. propose model which activities regulate cellular levels DGPP,
sci-hub.se 参考文章(47)
Sylvia Wagner Smith, Samuel B. Weiss, Eugene P. Kennedy, The enzymatic dephosphorylation of phosphatidic acids. Journal of Biological Chemistry. ,vol. 228, pp. 915- 922 ,(1957) , 10.1016/S0021-9258(18)70670-4
I N Fleming, S J Yeaman, Purification and characterization of N-ethylmaleimide-insensitive phosphatidic acid phosphohydrolase (PAP2) from rat liver Biochemical Journal. ,vol. 308, pp. 983- 989 ,(1995) , 10.1042/BJ3080983
Albert Haid, Mordechai Suissa, Immunochemical identification of membrane proteins after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Methods in Enzymology. ,vol. 96, pp. 192- 205 ,(1983) , 10.1016/S0076-6879(83)96017-2
B.G. Bhat, P. Wang, R.A. Coleman, Hepatic monoacylglycerol acyltransferase is regulated by sn-1,2-diacylglycerol and by specific lipids in Triton X-100/phospholipid-mixed micelles. Journal of Biological Chemistry. ,vol. 269, pp. 13172- 13178 ,(1994) , 10.1016/S0021-9258(17)36815-1
K R Morlock, J J McLaughlin, Y P Lin, G M Carman, Phosphatidate phosphatase from Saccharomyces cerevisiae. Isolation of 45- and 104-kDa forms of the enzyme that are differentially regulated by inositol. Journal of Biological Chemistry. ,vol. 266, pp. 3586- 3593 ,(1991) , 10.1016/S0021-9258(19)67835-X
A. Gomez-Muñoz, A. Martin, L. O'Brien, D.N. Brindley, Cell-permeable ceramides inhibit the stimulation of DNA synthesis and phospholipase D activity by phosphatidate and lysophosphatidate in rat fibroblasts. Journal of Biological Chemistry. ,vol. 269, pp. 8937- 8943 ,(1994) , 10.1016/S0021-9258(17)37058-8
M Bae-Lee, G M Carman, Regulation of yeast phosphatidylserine synthase and phosphatidylinositol synthase activities by phospholipids in Triton X-100/phospholipid mixed micelles. Journal of Biological Chemistry. ,vol. 265, pp. 7221- 7226 ,(1990) , 10.1016/S0021-9258(19)39102-1
Y P Lin, G M Carman, Kinetic analysis of yeast phosphatidate phosphatase toward Triton X-100/phosphatidate mixed micelles. Journal of Biological Chemistry. ,vol. 265, pp. 166- 170 ,(1990) , 10.1016/S0021-9258(19)40210-X
Y P Lin, G M Carman, Purification and characterization of phosphatidate phosphatase from Saccharomyces cerevisiae. Journal of Biological Chemistry. ,vol. 264, pp. 8641- 8645 ,(1989) , 10.1016/S0021-9258(18)81840-3
G.A. Jones, G Carpenter, The regulation of phospholipase C-gamma 1 by phosphatidic acid. Assessment of kinetic parameters. Journal of Biological Chemistry. ,vol. 268, pp. 20845- 20850 ,(1993) , 10.1016/S0021-9258(19)36862-0