Irradiation before and donor splenocyte infusion immediately after transplantation induce tolerance to lung, but not heart allografts in miniature swine

摘要: Solid organs may differ in their potential to induce and maintain a state of donor-specific tolerance. Previously, we induced stable immunological tolerance lung transplantation model miniature swine. Here, wished transfer this established protocol into heart swine. Heterotopic (HTX) was performed four, left-sided (LTX) seven minipigs from gender SLA mismatched donors. All recipients received non-myeloablative irradiation, donor splenocyte infusion intravenous pharmacologic immunosuppression for 28 postoperative days. All transplanted hearts were rejected within 95 days. In contrast, four animals the LTX group developed surviving beyond 500 days, three further 119, 239 360 days post transplantation. both groups peripheral blood leukocyte chimerism peaked 1h after reperfusion allograft. Importantly, early level significantly higher compared HTX remained detectable throughout entire observation period. In conclusion, lungs vary with pre-operative recipient irradiation co-transplantation. This could be due differential levels passenger chimerism. This article is protected by copyright. rights reserved.