Progesterone production by cultured luteal cells in the presence of bovine low- and high-density lipoproteins purified by heparin affinity chromatography.

摘要: The objectives of this study were to separate plasma lipoprotein particles based on the presence (low-density lipoproteins; LDL) or absence apolipoprotein B (high-density HDL) and compare abilities bovine LDL HDL stimulate progesterone production by luteal cells in culture. Plasma lipoproteins isolated ultracentrifugation separated into heparin affinity chromatography. Luteal cultures treated with cholesterol for 48 h d 3 culture (d 0 = day dissociation). Progesterone increased a dose-dependent manner increasing concentrations either cholesterol. There no differences ability produce progesterone. Because equally potent, experiment was designed investigate modified reconstituted without apolipoproteins production. Stimulation cell lysine-modified 70% unmodified LDL. phosphatidylcholine liposomes BSA containing 50 108% that obtained Evidence indicated apolipoprotein-free contained free but not esters stimulated