[BACTEC MGIT 960 system for drug susceptibility testing of Mycobacterium tuberculosis: a study using external quality assessment strains].

摘要: OBJECTIVE To evaluate the performance of BACTEC MGIT 960 system for drug susceptibility testing (MGIT AST) Mycobacterium tuberculosis to isoniazid, rifampin, streptomycin and ethambutol. DESIGN Fifty external quality assessment strains M. provided by Coordinating Centers WHO/ IUATLD were tested system, results compared with referee WHO/IUATLD Supranational Reference Laboratory Network (SRLN). RESULTS AND CONCLUSION Overall concordance rates obtained AST SRLN 97.3% four first-line drugs. Agreement particularly high (agreement rate over 97%), but somewhat lower ethambutol, which relates a sensitivity AST. Turnaround times from inoculation ranged 6 13 days median time 7 days; this contrasted three weeks proportion method using Middlebrook 7H10 agar, indicating that has potential consistently meet turnaround guidelines suggested Disease Control Prevention United States. These demonstrate fully automated is useful rapid diagnosis resistant tuberculosis.