A bioengineered microenvironment to quantitatively measure the tumorigenic properties of cancer-associated fibroblasts in human prostate cancer
作者： Ashlee K Clark , Anna V Taubenberger , Renea A Taylor , Birunthi Niranjan , Zhen Y Chea
DOI: 10.1016/J.BIOMATERIALS.2013.03.005
关键词:
摘要: Stromal-epithelial cell interactions play an important role in cancer and the tumor stroma is regarded as a therapeutic target. In vivo xenografting commonly used to study cellular not mimicked or quantified conventional 2D culture systems. To interrogate effects of (cancer-associated fibroblasts CAFs) on epithelia, we created bioengineered microenvironment using human prostatic tissues. Patient-matched CAFs non-malignant (NPFs) from men with moderate (Gleason 7) aggressive 8-9 castrate-resistant) prostate were cultured non-tumorigenic BPH-1 epithelial cells. Changes morphology, motility phenotype cells response NPFs analyzed immunofluorescence quantitative morphometric analyses. The matrix protein gene expression CAFs, proven tumorigenicity vivo, had significantly different profile proteins compared patient matched NPFs. co-culture (but NPFs), more invasive, elongated increased directed pattern migration. tumors CRPC) quantitatively grade CAFs. Overall, our provides novel 3D vitro platform systematically investigate progression.
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