Structure of the microbial flora associated with periodontal health and disease in man. A light and electron microscopic study.

摘要: Teeth slated for extraction were evaluated with respect to their periodontal status and classified accordingly into five categories; namely normal, gingivitis, periodontitis, periodontosis postperiodontosis. After processing, one approximal surface of each tooth was sampled at various levels in an apico-occlusal direction light electron microscopic study the associated bacterial flora. In normal samples, flora consisted a relatively thin, adherent layer confined enamel surface. The cells predominantly coccoid shape cell wall features compatible those Gram-positive organisms. Isolated filamentous or branching forms some Gram-negative bacteria noted on more apical portion layer. No flagellated spirochetes present. Gingivitis samples yielded voluminous complex supragingival ultrastructure. These also contained corncob formations deposits, within sulcus bottom. Supragingival deposits periodontitis similar observed gingivitis. subgingival fewer root concomitant increase population cells, as well spirochetes. tissue side generally exhibited distinctive concentration "test-tube brush" formations, medium size, assorted types peculiar this region. A transitional separated supra- from microbial population. Periodontosis had sparse, unique electron-dense, lobulated cuticular deposit covered majority studied. Postperiodontosis much group. results suggest that (1) certain may be state health; (2) different is varying degrees disease; (3) structure composition differs markedly flora; (4) exception periodontis, alterations disease increases inseverity parallel changes described previously collected artificial crowns during experimentally induced use expressions "microbial flora" population" considered preferable terms "plaque", "materia alba", "debris" reference microbiota gingival