Desialylation of Spermatozoa and Epithelial Cell Glycocalyx Is a Consequence of Bacterial Infection of the Epididymis.

摘要: Urinary tract infections caused by uropathogenic Escherichia coli (UPEC) pathovars belong to the most frequent in humans. In men, pathogens can also spread genital via continuous ductal system, eliciting bacterial prostatitis and/or epididymo-orchitis. Antibiotic treatment usually clears acute epididymitis; however, fertility of patients be permanently impaired. Because a premature acrosome reaction was observed an UPEC epididymitis mouse model, and sialidases on sperm surface are considered activated proteases acrosome, we aimed investigate whether alterations sialome epididymal spermatozoa surrounding epithelial cells occur during infection. UPEC-elicited mice, substantial loss N-acetylneuraminic acid residues detected using combined laser microdissection/HPLC-ESI-MS analysis. support, reduction sialic bound documented men with recent history E. coli-associated epididymitis. vitro, such induced release from human effectively counteracted sialidase inhibitor. These findings strongly suggest remodeling glycocalyx endogenous after