Phenotypic and molecular characteristics of Escherichia coli isolated from aquatic environment of Bangladesh.

摘要: Pathogenic Escherichia coli remains important etiological agent of infantile diarrhea in Bangladesh. Previous studies have focused mostly on clinical strains, but very little is known about their presence aquatic environments. The present study was designed to characterize potentially pathogenic E. isolated between November 2001 and December 2003 from environments 13 districts Serotyping 96 randomly selected strains revealed O161 be the predominant serotype (19%), followed by O55 O44 (12% each), 11% untypable. Serotype-based pathotyping 47%, 30%, 6% belong EPEC, ETEC, EHEC pathotypes, respectively. majority 160 tested were resistant commonly used antimicrobial agents. Plasmid pro-filing showed a total 17 different bands ranging 1.3 40 kb. However, 35% did not contain any detectable plasmid, implying no correlation plasmid drug resistance. Although virulence gene profiling 97 (61%) harbor encoding heat-stable enterotoxin (ST), 2 for Shiga toxin (Stx), none heat-labile (LT), serotype-based fully supported this profiling. A dendrogram derived PFGE patterns 22 three serogroups indicated two major clusters, one containing mainly serogroup other O8. Three identical profiles belonging distinct areas, which may epidemiological significance. Finally, it concluded that useful origin; however, precise enough reliably identifying environmental as diarrheagenic.