Short curcumin treatment modulates oxidative stress, arginase activity, aberrant crypt foci, and TGF-β1 and HES-1 transcripts in 1,2-dimethylhydrazine-colon carcinogenesis in mice.

摘要: This study investigated the effect of short curcumin treatment, a natural antioxidant on 1,2-dimethylhydrazine (DMH)-induced aberrant crypt foci (ACF) in mice. The incidence was 100%, with 54 ± 6 per colon, 10 weeks after first DMH injection and reached 67 12 colon weeks. A high level undifferentiated goblet cells weak apoptotic activity were shown dysplastic ACF. morphological alterations colonic mucosa associated to severe oxidative stress ratio 43% increase malondialdehyde vs. 36% decrease GSH. also increased inducible nitric synthase (iNOS) mRNA transcripts (250%), nitrites (240%) arginase (296%), leading nitrosative cell proliferation. Curcumin starting at week post-DMH for 14 days, reduced number ACF (40%), iNOS expression (25%) (73%), improved redox status by approximately 46%, compared DMH-treated Moreover, induced apoptosis without restoring differentiation. Interestingly, parallel TGF-β1 HES-1 (42% 26%, respectively). In conclusion, protective driven reduction stress. up regulation suggests time, potential interplay between these signalling pathways chemoprotective mechanism curcumin.