Target-selective photo-degradation of a sialyl Lewis a (sLea) conjugate and photo-cytotoxicity against sLea positive cancer cells using an anthraquinone-antibody hybrid
作者： Daisuke Takahashi , Takashi Nagao , Shota Sotokawa , Kazunobu Toshima
DOI: 10.1039/C6MD00167J
关键词:
摘要: A purpose-designed anthraquinone–monoclonal antibody (anti-sialyl Lewis (sLea) mAb) hybrid 6 selectively bound to and effectively degraded the target glycoprotein, HSA (human serum albumin)–sLea conjugate 4. Degradation was achieved using long wavelength UV light irradiation in absence of any additives under neutral conditions. Furthermore, exhibited selective photo-cytotoxicity against sLea positive cancer cells A431 WiDr only upon photo-irradiation.
rsc.org
elsevier.com
sci-hub.se 参考文章(32)
Thomas Spector, Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein. Analytical Biochemistry. ,vol. 86, pp. 142- 146 ,(1978) , 10.1016/0003-2697(78)90327-5
Yang Chen, Guanghua Liu, Lifang Guo, Hui Wang, Yan Fu, Yongzhang Luo, Enhancement of tumor uptake and therapeutic efficacy of EGFR-targeted antibody cetuximab and antibody-drug conjugates by cholesterol sequestration. International Journal of Cancer. ,vol. 136, pp. 182- 194 ,(2015) , 10.1002/IJC.28950
Victor Ginsburg, Zenon Steplewski, Hilary Koprowski, John L. Magnani, Identification of the gastrointestinal and pancreatic cancer-associated antigen detected by monoclonal antibody 19-9 in the sera of patients as a mucin Cancer Research. ,vol. 43, pp. 5489- 5492 ,(1983)
J L Magnani, B Nilsson, M Brockhaus, D Zopf, Z Steplewski, H Koprowski, V Ginsburg, A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II. Journal of Biological Chemistry. ,vol. 257, pp. 14365- 14369 ,(1982) , 10.1016/S0021-9258(19)45389-1
Umashankar K Ballehaninna, Ronald S Chamberlain, The clinical utility of serum CA 19-9 in the diagnosis, prognosis and management of pancreatic adenocarcinoma: An evidence based appraisal Journal of gastrointestinal oncology. ,vol. 3, pp. 105- 119 ,(2012) , 10.3978/J.ISSN.2078-6891.2011.021
Robbert J. de Haas, Dennis A. Wicherts, Eduardo Flores, Michel Ducreux, Francis Lévi, Bernard Paule, Daniel Azoulay, Denis Castaing, Antoinette Lemoine, René Adam, Tumor marker evolution : comparison with imaging for assessment of response to chemotherapy in patients with colorectal liver metastases Annals of Surgical Oncology. ,vol. 17, pp. 1010- 1023 ,(2010) , 10.1245/S10434-009-0887-5
J. Magnani, M Brockhaus, D. Smith, V Ginsburg, M Blaszczyk, K. Mitchell, Z Steplewski, H Koprowski, A monosialoganglioside is a monoclonal antibody-defined antigen of colon carcinoma. Science. ,vol. 212, pp. 55- 56 ,(1981) , 10.1126/SCIENCE.7209516
S.A. Bobrovnik, Determination of antibody affinity by ELISA. Theory Journal of Biochemical and Biophysical Methods. ,vol. 57, pp. 213- 236 ,(2003) , 10.1016/S0165-022X(03)00145-3
Pei-Ching Lin, Jen-Kou Lin, Chun-Chi Lin, Huann-Sheng Wang, Shung-Haur Yang, Jeng-Kai Jiang, Yuan-Tzu Lan, Tzu-Chen Lin, Anna Fen-Yau Li, Wei-Shone Chen, Shih-Ching Chang, Carbohydrate antigen 19-9 is a valuable prognostic factor in colorectal cancer patients with normal levels of carcinoembryonic antigen and may help predict lung metastasis International Journal of Colorectal Disease. ,vol. 27, pp. 1333- 1338 ,(2012) , 10.1007/S00384-012-1447-1
Yukari Imai, Shingo Hirono, Haruka Matsuba, Tomohiro Suzuki, Yuka Kobayashi, Hirokazu Kawagishi, Daisuke Takahashi, Kazunobu Toshima, Degradation of target oligosaccharides by anthraquinone-lectin hybrids with light switching. Chemistry-an Asian Journal. ,vol. 7, pp. 97- 104 ,(2012) , 10.1002/ASIA.201100586