Hypoxic tumor microenvironments reduce collagen I fiber density.

摘要: Although the mechanisms through which hypoxia influences several phenotypic characteristics such as angiogenesis, selection for resistance to apoptosis, radiation and chemotherapy, increased invasion metastasis are well characterized, relationship between tumor components of extracellular matrix (ECM) is relatively unexplored. The collagen I (Col1) fiber solid tumors major structural part ECM. Col1 density can increase initiation, progression, metastasis, with cancer cell occurring along radially aligned fibers. Here we have investigated influence on in breast prostate models. Second harmonic generation (SHG) microscopy was used detect differences volume hypoxic normoxic regions. Hypoxic regions were detected by fluorescence microscopy, using derived from human lines stably expressing enhanced green fluorescent protein (EGFP) under transcriptional control response element. In-house analysis software quantitatively analyze SHG images. Normoxic exhibited a dense mesh In contrast, fewer structurally altered fibers EGFP-expressing Microarray gene expression analyses identified lysyl oxidase reduced some metalloproteases compared cells. These results suggest that mediates restructuring tumors, may impact delivery macromolecular agents dissemination