Erythrocyte-Associated Transients in Capillary PO2 in the Rat Spinotrapezius Muscle During Hemodilution with Hespan and a Hemoglobin-Based Oxygen Carrier

摘要: ERYTHROCYTE-ASSOCIATED TRANSIENTS IN CAPILLARY PO2 THE RAT SPINOTRAPEZIUS MUSCLE DURING HEMODILUTION WITH HESPAN AND A HEMOGLOBIN-BASED OXYGEN CARRIER By Matthew C. Barker thesis submitted in partial fulfillment of the requirements for degree Master Science at Virginia Commonwealth University University, 2005 Advisor: Roland N. Pittman, Ph.D. Department Physiology Hemoglobin-based oxygen carriers use as transfusion fluids have emerged a leading technology directed stemming shortages safe blood supply and providing readily available resuscitation fluid various trauma situations. The purpose this investigation was to determine effects isovolemic hernodilution with Hespan hemoglobin-based carrier (HBOC) on erythrocyte-associated transients (EATs) capillary PO2. particulate nature flow capillaries, when observed from stationary observation point, results fluctuations alternating red cells plasma gaps move through detection region. Therefore, experimental methods which provided necessary temporal spatial resolution required make such measurements, EATs can be corresponding determined. spinotrapezius muscle sixteen Sprague-Dawley rats exteriorized intravital microscopy measurements capillaries. Capillary measured using Pd-porphyrin phosphorescence quenching microcopy. hemodiluents used hemodilution included Hespan, non-oxygen carrying expander, OxyglobinB, HBOC. Two steps were performed, reducing systemic hematocrit an average 27.5% after first step 13.5% second step. Results showed that rat significant differences occurring between cell gap under control conditions, Oxyglobin one, two. This study concludes are observable POz relating muscle. Furthermore, it concluded HBOC caused decrease transients, well general In addition, best conditions two Hespan.