Establishment and characterization of turbine oil-degrading bacterial consortia

作者: Reia Hosokawa , Naofumi Sakaguchi , Hidetoshi Okuyama

DOI: 10.1016/J.IBIOD.2010.06.006

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摘要: A microbial consortium capable of degrading turbine oil (TuO) consisting mainly recalcitrant branched and polycyclic alkanes, has been established using a sludge sample collected from an oil-water separation tank hydraulic plant, by repeated enrichment cultivation. When this consortium, named the tank-2 was cultivated in minimal salts medium containing 0.5% TuO, it degraded approximately 90% TuO at 30 °C under shaking 160 rpm five days. During cultivation, formed cell aggregates. Fourteen bacterial species were isolated consortium. Although mixtures all strains (IM) predominant selected IM (PM) 60% maximally seven days after enrichment, degradation rate not maintained. The decreased PM recovered inoculation with mixture (AM) isolates involved aggregate formation suggesting that aggregates is important factor for consortia. Interestingly, only observed when cyclic alkanes added to as source carbon. surface hydrophobicity significantly high (77.6%). These results suggest growth bacteria can be induced fraction facilitates cells form contact components uptakes these components.

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