Bone morphogenetic protein-2b stimulation of growth and osteogenic phenotypes in rat osteoblast-like cells: Comparison with TGF-β1
作者： Theresa L. Chen , Rebecca L. Bates , Andrew Dudley , Glenn R. Hammonds , Edward P. Amento
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摘要: The biologic effects of recombinant human bone morphogenetic protein-2b (BMP-2b = BMP-4) were studied and compared with transforming growth factor-beta 1 (TGF-beta 1) in fetal rat osteoblast-like (ROB) cells. Similar to the TGF-beta 1, BMP-2b stimulated DNA collagen synthesis as well protein accumulation. Unlike which inhibited alkaline phosphatase activity, enhanced enzyme activity eight-to ninefold over control level. present study demonstrates direct actions on bone-associated cells stimulate osteogenic phenotypes vitro provides a cellular mechanism for induction formation by vivo.
sci-hub.se 参考文章(33)
E. Ozkaynak, D. C. Rueger, E. A. Drier, C. Corbett, R. J. Ridge, T. K. Sampath, H. Oppermann, OP-1 cDNA encodes an osteogenic protein in the TGF-beta family. The EMBO Journal. ,vol. 9, pp. 2085- 2093 ,(1990) , 10.1002/J.1460-2075.1990.TB07376.X
F P Luyten, N S Cunningham, S Ma, N Muthukumaran, R G Hammonds, W B Nevins, W I Woods, A H Reddi, Purification and partial amino acid sequence of osteogenin, a protein initiating bone differentiation. Journal of Biological Chemistry. ,vol. 264, pp. 13377- 13380 ,(1989) , 10.1016/S0021-9258(18)80003-5
M. Centrella, T.L. McCarthy, E. Canalis, Transforming growth factor beta is a bifunctional regulator of replication and collagen synthesis in osteoblast-enriched cell cultures from fetal rat bone. Journal of Biological Chemistry. ,vol. 262, pp. 2869- 2874 ,(1987) , 10.1016/S0021-9258(18)61587-X
B R Genge, G R Sauer, L N Wu, F M McLean, R E Wuthier, Correlation between loss of alkaline phosphatase activity and accumulation of calcium during matrix vesicle-mediated mineralization. Journal of Biological Chemistry. ,vol. 263, pp. 18513- 18519 ,(1988) , 10.1016/S0021-9258(19)81388-1
G.W. Cyboron, R.E. Wuthier, Purification and initial characterization of intrinsic membrane-bound alkaline phosphatase from chicken epiphyseal cartilage. Journal of Biological Chemistry. ,vol. 256, pp. 7262- 7268 ,(1981) , 10.1016/S0021-9258(19)68956-8
T K Sampath, J E Coughlin, R M Whetstone, D Banach, C Corbett, R J Ridge, E Ozkaynak, H Oppermann, D C Rueger, Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-beta superfamily. Journal of Biological Chemistry. ,vol. 265, pp. 13198- 13205 ,(1990) , 10.1016/S0021-9258(19)38285-7
J.R. Farley, J.L. Ivey, D.J. Baylink, Human skeletal alkaline phosphatase. Kinetic studies including pH dependence and inhibition by theophylline. Journal of Biological Chemistry. ,vol. 255, pp. 4680- 4686 ,(1980) , 10.1016/S0021-9258(19)85548-5
M. R. Urist, Bone: Formation by Autoinduction Science. ,vol. 150, pp. 893- 899 ,(1965) , 10.1126/SCIENCE.150.3698.893
Bryan Sykes, Barry Puddle, Martin Francis, Roger Smith, The estimation of two collagens from human dermis by interrupted gel electrophoresis Biochemical and Biophysical Research Communications. ,vol. 72, pp. 1472- 1480 ,(1976) , 10.1016/S0006-291X(76)80180-5
Rik Derynck, Julie A. Jarrett, Ellson Y. Chen, Dennis H. Eaton, John R. Bell, Richard K. Assoian, Anita B. Roberts, Michael B. Sporn, David V. Goeddel, Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells. Nature. ,vol. 316, pp. 701- 705 ,(1985) , 10.1038/316701A0