Optimal lymphocytic choriomeningitis virus sequences restricted by H-2Db major histocompatibility complex class I molecules and presented to cytotoxic T lymphocytes.
作者： J E Gairin , H Mazarguil , D Hudrisier , M B Oldstone
DOI: 10.1128/JVI.69.4.2297-2305.1995
关键词:
摘要: Infection with lymphocytic choriomeningitis virus induces the generation of CD8+ cytotoxic T lymphocytes (CTL). In H-2b mouse, this cellular immune response is directed against three viral structural epitopes (GP1, GP2, and NP) presented by major histocompatibility complex (MHC) class I H-2Db molecules. This study was undertaken to delineate which sequence each these optimal for MHC binding CTL recognition. The first step synthesize relevant peptides truncated at N or C terminus flanking crucial H-2Db-anchoring Asn residue in position 5. These were then tested (i) their properties two H-2Db-specific assays viable cells (upregulation expression on surface RMA-S competition Db-restricted peptide 125I-gp276-286 T2-Db cells) (ii) abilities sensitize target lysis vitro. For antigenic presentation, all required MHC-anchoring 5 sequences. results clearly unambiguously delineated lengths options third. NP appeared as a conventional 9-amino-acid (aa)-long peptide, np396-404 (FQPQNGQFI). GP2 defined longer (11 aa), gp276-286 (SGVENPGGYCL). Characterization GP1 epitope more complex: 9-aa-long gp33-41 (KAVYNFATC) carboxyl-extended 11-aa-long gp33-43 (KAVYN FATCGI) both established possible sequences depending cell line used test lysis.
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