Fluorescence resonance energy transfer imaging microscopy.

摘要: Publisher Summary This chapter describes the fluorescence resonance energy transfer (FRET) imaging microscopy. RET is a process by which donor fluorophore (D) in its excited state may excitation to nearby chromophore (acceptor, A). The nonradiative (not mediated photon) and achieved through dipole–dipole interactions. In principle, for such occur, several major criteria must be satisfied. First, molecule have an emission spectrum that overlaps absorption of acceptor molecule. second criterion needs satisfied molecules within 10–100 A each other. efficiency between falls off as sixth power distance separating molecules. Another met lifetime sufficient duration allow FRET occur. Continued research this area needed determine optimal approaches measurement development more refined algorithms analysis data.