Lyophilized Silk Sponges: A Versatile Biomaterial Platform for Soft Tissue Engineering.
作者： Jelena Rnjak-Kovacina , Lindsay S. Wray , Kelly A. Burke , Tess Torregrosa , Julianne M. Golinski
DOI: 10.1021/AB500149P
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摘要: We present a silk biomaterial platform with highly tunable mechanical and degradation properties for engineering regeneration of soft tissues such as, skin, adipose, neural tissue, elasticity in the kilopascal range. Lyophilized sponges were prepared under different process conditions effect molecular weight, concentration crystallinity on 3D scaffold formation, structural integrity, morphology, properties, cell interactions vitro vivo studied. Tuning weight distribution (via degumming time) allowed formation stable, porous, scaffolds that held form concentrations as low 0.5% wt/v. Mechanical function was driven by beta-sheet content. supported adhesion mesenchymal stem cells throughout scaffolds, proliferation vitro, infiltration re...
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