Prokineticin 2 facilitates mechanical allodynia induced by α,β-methylene ATP in rats.

摘要: Prokineticin 2 (PK2), a new chemokine, causes mechanical hypersensitivity in the rat hind paw, but little is known about molecular mechanism. Here, we have found that ionotropic P2X receptor essential to allodynia induced by PK2. First, intraplantar injection of high dose (3 or 10 pmol) PK2 significantly increased paw withdrawal response frequency (%) innocuous stimuli (mechanical allodynia). And was prevented co-administration TNP-ATP, selective antagonist. Second, although low (0.3 1 itself did not produce an allodynic response, it facilitated evoked α,β-methylene ATP (α,β-meATP). Third, concentration-dependently potentiated α,β-meATP-activated currents dorsal root ganglion (DRG) neurons. Finally, receptors and intracellular signal transduction were involved potentiation α,β-meATP-induced currents, since blocked antagonist PKRA PKC inhibitor GF 109203X. These results suggested α,β-meATP through mechanism sensitization cutaneous expressed nociceptive nerve endings.